Layout type:

Define whether the samples are placed onto the plate in groups or in rows, and whether they will be ordered horizontally or vertically. 

We strongly recommend organizing samples by groups, as this makes the pipetting scheme easy to follow. 


Plate size:

The plate size can be modified, depending on the qPCR machine different plates/sample rings are used. 36, 72, 96 and 384 well plates can be used.


Color mode:

The color highlights which appear on the software interface and the protocols can be modified to use a scheme which is preferred by the user.


Replicates:

Define whether to perform quantitative analysis using duplicates or triplicates. 

GenDx recommends the use of triplicates, as low concentration DNA samples may exhibit stochastic variance in Cq.