Multiplexed sample genotyping is performed using a panel of 39 non-repetitive assays targeting polymorphic sites located on 18 different chromosomes with 3 qPCR assays per well. 

KMRtype Genotyping Kits (Core set) contain two times 10 tubes of assay mixtures, KMRtype Mixes 01 - 10: each mix contains 3 qPCR assays.

KMRtype Genotyping Kits (Extended set) contain two times 3 tubes of assay mixtures, KMRtype Mixes 11 - 13: each mix contains 3 qPCR assays.

The qPCR assays in each well have probes in colors different from each other. 

When using the Core set, the 10 mixes are dispensed into the first 10 wells (Mix 1 into well 1, Mix 2 into well 2, etc.) of a 96-well optical plate.

The KMRassay Reference Assay is dispensed into wells 11 and 12.

Well 11 serves as a positive control and well 12 is a no-template control (NTC) reaction.





KMRengine identifies informative markers for up to 8 samples in a single 96-well plate.