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NGS-Turbo®

Complete solution for Oxford Nanopore sequencing

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Benefits

  • Amplification & library preparation
  • DNA to results ~2.5h
  • 1 sample per run

Description

With a turnaround time of less than three hours (single sample) and an easy and robust workflow, NGS-Turbo® is designed for HLA typing in situations where time is a critical factor.

The NGS-TurboAmp kit offers 11 HLA loci using a whole-gene approach. The extremely quick 1-tube library preparation with NGS-TurboPrep requires no fragmentation and can be safely paused at any time. After nanopore sequencing the data can be directly analyzed with NGSengine®-Turbo.

Available as RUO.

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Product Status Reactions Catalog

NGS-Turbo®Amp

Components

NGS-Turbo®Amp

 RUO 12 9302210 Favorite

NGS-Turbo®Prep

Components

NGS-Turbo®Prep

 RUO 12 9302220 Favorite

Specifications

High-resolution HLA typing can be performed on multiple platforms, however, options are limited when time is of the essence. The MinION Mk1B and GridION sequencers from ONT are compatible with NGS-Turbo and provide long-read sequencing within a short timeframe, taking as little as 20 minutes for one sample.

With NGS-Turbo®, the turnaround time for high-resolution HLA typing is only 3 hours, from blood to typing result.

 

Amplification of HLA loci

NGS-TurboAmp offers fast amplification of all 11 HLA loci (HLA-A, B, C, DQA1, DQB1, DPA1, DPB1, DRB1 and DRB3/4/5) using a whole-gene approach and results in high resolution.

Library preparation

With NGS-TurboPrep, library preparation can be performed in just 30 minutes. No fragmentation is required and intermission is possible at any time point.

Sequencing & data analysis

Finally, the sample can be sequenced on Oxford Nanopore Technologies sequencing devices and the data can be directly analyzed and typed with NGSengine-Turbo.

Compatibility

NGS-Turbo is compatible with R10.4.1 flow cells, and can be sequenced on MinION Mk1B and GridION sequencers, delivering rapid results in as little as 20 minutes per sample. The assay is to be used with compatible consumables from ONT, including SQK-LSK114 and EXP-AUX003.
NGS-Turbo has been validated to be compatible with MinKNOW versions 23.04.3, 23.11.7, and 24.06.5.

Downloads

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