GenDx > GenDx Products > NGS HLA Typing > Library preparation > NGS-ProntoPrep®

NGS-ProntoPrep®

Library preparation for ONT

Benefits

  • Barcoded library prep for ONT (RUO)
  • Fast workflow in 1.5–2 hours
  • 1 to 96 samples

Description

NGS-ProntoPrep offers a complete, easy-to-use barcoded library preparation solution specifically designed for high-resolution HLA typing on Oxford Nanopore Technologies (ONT) devices. Designed for speed and accuracy, the workflow takes just 1.5 to 2 hours and is easily scalable for 1 to 96 samples.

For a complete ONT workflow, simply amplify your DNA with NGSgo-ProntoAmp or NGSgo-ProntoFLX, prepare your library with NGS-ProntoPrep, sequence, and analyze your data with NGSengine.

NGS-ProntoPrep is available as RUO.

Do you have questions or want to arrange a demo?

Contact our Sales Team

See specifications
Top Catalog Specifications Downloads

Catalog

Contact us to order
Or contact your local distributor

Filter

  • Status

1 Items

Product Status Reactions Catalog

NGS-Pronto®Prep

Components

Nuclease-free water

AMPure XP beads

End Prep Mix

Ligase Mix

Binding Solution

Elution Buffer

Wash Buffer

10x Ligation Buffer

Ligase Enzyme

Barcode Plate 1

 

 

 RUO 96 9303623 Favorite

Specifications

Library preparation

The library preparation process is highly efficient and performed entirely at room temperature, allowing for a flexible workflow that can be easily paused. Depending on the number of samples, the procedure takes just 1.5 to 2 hours to complete.

Because the protocol skips fragmentation, it yields long, intact amplicons that enable full phasing. The kit is highly scalable, accommodating 1 to 96 samples per run, with 96 unique barcodes available. Sample pooling is performed conveniently in the presence of beads, and the kit supports a maximum of 12 libraries (1 to 96 samples per library).

Compatibility

Compatible with R10.4.1 flow cells, NGS-Pronto can be sequenced on MinION Mk1B, Mk1D, and GridION sequencers from ONT, delivering rapid results in as little as 10-15 minutes per sample. The assay is to be used with compatible consumables from ONT, including EXP-NBA114 and EXP-AUX003.

NGS-Pronto has been validated to be compatible with MinKNOW versions 23.04.3, 23.11.7, 24.06.5, 24.11.8, 24.11.10, and 25.05.14.

Amplification and analysis

The NGS-ProntoPrep kit is designed to seamlessly follow NGSgo-ProntoAmp or NGSgo-ProntoFLX amplification. The resulting data is optimized for fast, accurate interpretation using NGSengine®.

Turnaround time for 12 samples < 8 hours:

Downloads

Log in to browse the downloads.