GenDx > GenDx Products > NGS HLA Typing > Amplification > NGS-Pronto®

NGS-Pronto®

Complete solution for Oxford Nanopore sequencing

Benefits

  • Amplification & barcoded library preparation
  • DNA to results < 8h
  • Up to 96 samples per run

Description

Get ready for the future of HLA typing with NGS-Pronto, our solution designed for Oxford Nanopore Technologies sequencing devices.

NGSgo-ProntoAmp offers amplification of 11 HLA loci using a whole-gene approach, resulting in at least a 3-field resolution. NGS-ProntoPrep offers a complete barcoded library preparation for sequencing on ONT devices. After nanopore sequencing, the data can be directly analysed with NGSengine®-Turbo.

NGSgo-ProntoAmp and NGS-ProntoPrep are available as RUO.

The NGSgo-ProntoAmp amplification is also compatible with the NGSgo Library Full Kit for sequencing on Illumina systems!

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NGS-Pronto®Prep

Components

Nuclease-free water

AMPure XP beads

End Prep Mix

Ligase Mix

Binding Solution

Elution Buffer

Wash Buffer

10x Ligation Buffer

Ligase Enzyme

Barcode Plate 1

 

 

 RUO 96 9303623 Favorite

NGSgo®-ProntoAmp

Components

Mix 1

Mix 2

Mix 3

 RUO 96 9303613 Favorite

Specifications

Amplification & Library Preparation

Covering 11 HLA loci using a comprehensive whole-gene approach, the fast thermal cycling program is completed in just 90 minutes. Library preparation is equally efficient, performed entirely at room temperature, and easily paused as needed. Additionally, there is no fragmentation – our process yields long, intact amplicons to further achieve full phasing. Depending on the sample number, library prep takes just 1.5 to 2 hours.

Compatibility

Compatible with R10.4.1 flow cells, NGS-Pronto can be sequenced on MinION Mk1B and GridION sequencers from ONT, delivering rapid results in as little as 15 minutes per sample. The assay is to be used with compatible consumables from ONT, including EXP-NBA114 and EXP-AUX003.
NGS-Pronto has been validated to be compatible with MinKNOW versions 23.04.3, 23.11.7, and 24.06.5.

Data analysis

Streamline your analysis with NGSengine-Turbo, our user-friendly software tailored for ONT data. With a fast analysis time of approximately 5 minutes per sample, you’ll quickly have the results you need.

NGS-Pronto® turnaround time

*time dependent on number of samples

Downloads

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