KMRtrack®

Specifications

The GenDx Chimerism Monitoring workflow

Advantages of KMRtype & KMRtrack

• Use of 39 markers strategically placed over 17 chromosomes: This way, the chance of finding an informative marker is increased.

Chromosomal location of KMRtype & KMRtrack markers

• Multiple donor analysis: With our highly sensitive system multiple donors can be traced simultaneously. With the increased use of double cord blood donations this feature improves the opportunities for highly sensitive and longitudinal monitoring.

• Up to 8 samples per genotyping experiment: With a multiplexed assay the number of reactions and reagents needed per sample is minimized, enabling an experimental set-up of up to 8 samples per run (when applying a 96-well plate and the KMRtype Core kit). Samples from both recipient and donor can be analyzed in separate experiments, providing additional flexibility.

• Compatible with most common qPCR machines: ABI (ViiA7 v1.2.4 / 7500 Fast v2.0.6 / 7500 v1.3.1/1.4/2.0.2/2.0.6/2.3), QuantStudio (5 v1.2 / 6 v1.2, v1.3 / 7 v1.2 / 12K v1.2 / DX v1.2), BioRad (CFX 96 v1.6/3.0/3.1).

Kit content RUO version

The Core and Extended kits for KMRtype and KMRtrack differ in number of assays that they contain. The Core kit offers 30 assays, while an additional 9 assays can be found in the Extended kit, which can be applied in cases where no informative marker was obtained with the Core kit. You can apply both the Core and Extended kit at the same time, genotyping your samples for 39 assays in one go. There are also kits available that include reference assay, buffer and enzyme. More info about the kit content can be found in instructions for use.

Kit content IVD version

The Core and Extended kits for KMRtype and KMRtrack contain all 39 assays, reference assay, buffer, and enzyme.

To be supplied by the user:

• Pipettes and tips (hydrophobic filters)
• Ice or cooling block
• Optical 96-wells reactions plates
• Eppendorf tubes
• PCR tubes, strips or plates
• Optical adhesive seals
• Micro centrifuge
• Microtiter Plate Centrifuge
• Vortex
• MilliQ water
• Compatible qPCR machine