Quality metrics
The 'Quality metrics' tab displays the same quality metrics as shown in the overview screen.
- Read length (median): The median read length of the accepted reads.
- Read depth: The number of reads covering a single position.
- Coverage: The percentage of positions covered by at least one read.
- QV (median): The median Quality Value of all analyzed positions of all reads.
- Noise: The percentage of basecalling errors produced during sequencing.
Median: the median noise over all analyzed positions.
Maximum: The noise percentage at the position with the highest noise level.
- Analyzed: The percentage of the amplicon used for analysis.
- Delta signal to noise: The difference between the highest noise position and the lowest heterozygous position in %.
A high number indicates high quality data.
Negative numbers can occur when noise levels are high.
- Second allele: The percentage of the heterozygous positions with the lowest abundance.
- Phased regions: The number of phased regions within the locus.
- Question mark positions: A list of gDNA positions of which it could not be determined whether they are heterozygous or homozygous.