Amplification Strategies:


NGSgo®-ProntoAmp 

Principle:

NGSgo-ProntoAmp consists of reagents that enable the multiplexed amplification of the following Human Leukocyte Antigen (HLA) genes:

 

1 except for part of intron 1

2 HLA-DRB1*04 is added separately to support strong amplification if this allele is present. Coverage of the locus remains whole gene upon pooling of mixes.


Coverage: 

    


NGSgo®-TurboAmp 

Principle: NGS-Turbo consists of reagents to amplify Human Leukocyte Antigen (HLA) genes, and reagents for library preparation and sequencing on Oxford Nanopore Technologies sequencing devices. NGS-Turbo is designed for processing one DNA sample at a time. The reagents enable the multiplexed amplification of the following HLA genes:


1 except for part of intron 1 

2 HLA-DRB1*04 is added separately to compensate for potential imbalanced amplifications if this allele is present. Coverage of the locus remains whole gene upon pooling of mixes.


Coverage: 


NGSgo®-MX11-3 

Principle: NGSgo-MX11-3 consists of PCR amplification reagents to amplify Human Leukocyte Antigen (HLA) genes. The reagents enable the multiplexed amplification of the following HLA genes:


Coverage: 


NGSgo®-MX6-1

Principle: NGSgo-MX6-1 consists of PCR amplification reagents to amplify Human Leukocyte Antigen (HLA) genes. The reagents enable the multiplexed amplification of the following HLA genes: HLA-A (whole gene), HLA-B (whole gene), HLA-C (whole gene), HLA-DRB1 (exon 2 to exon 3), HLA-DQB1 (exon 2 to part of exon 4), and HLA-DPB1 (exon 2 to exon 5).


Coverage: 


NGSgo®-AmpX v2/AmpX v3

Principle: NGSgo-AmpX v2 is a qualitative in vitro diagnostic kit, intended for the amplification of the following genes in human genomic DNA specimens: 

• HLA Class I: HLA-A, HLA-B, HLA-C 

• HLA Class II: HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, HLA DQA1, HLA DQB1, HLA-DPA1, HLA-DPB1


Coverage: