We are continuously striving to improve our NGSgo workflow, both in terms of robustness and speed while maintaining the highly reliable results you are used to. To make the workflow faster we have modified several steps from amplification to data analysis.
based on 10 samples tested with NGSgo®-MX6-1 in combination with Illumina nano flow cell (2x150bp)
One-Day -Workflow video
Multiplex amplification
NGSgo-MX6-1 features six loci in one tube for the amplification of HLA-A, -B, -C, -DRB1, -DQB1, and -DPB1.
Ready-to-use PCR mastermix
Pipetting steps are minimized by using the GenDx-LongMix enzyme mastermix, which is included in the NGSgo-MX6-1 kit. You combine just four components: mastermix, primer mix, DNA, and water.
Reliable quality
We have shortened and simplified the workflow, while maintaining the highly reliable results you are used to. The NGSgo primer design has been fine-tuned and optimized for over a decade and is extremely accurate and robust. The advanced design means well-balanced alleles, low noise, and high-quality results.
Amplicon generation
NGSgo®-MX6-1 offers multiplexed amplification primers for HLA-A, B, C, DRB1, DQB1,DPB1
GenDx-LongMix PCR mastermix minimizes pipetting steps
Library preparation
NGSgo®-IndX plates Simplify your workflow with our new IndX plates
Data analysis
NGSignition automatically starts analyzing your sequencing without user intervention
Results
NGSengine® delivers your typing results with the highest accuracy in the fastest manner
NGSgo®-MX6-1 is both available as CE-marked IVD and RUO. GenDx-LongMix and NGSgo®-IndX plates are RUO. (RUO: Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease).
For questions about our One-Day-Workflow contact us at +31 (0)30 252 3799 (NL) or +1(312)815 5006 (USA) or via e-mail at support@gendx.com