GenDx > GenDx Products > NGS HLA Typing > Amplification > NGSgo®-MX11-3

NGSgo®-MX11-3

Multiplexed HLA amplification 11 loci

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Benefits

  • Multiplex amplification
  • Whole-gene coverage
  • RUO & CE-IVD (IVDR)

Description

NGSgo-MX11-3 features amplification primers for eleven loci, multiplexed in three tubes. The three-tube design results in lower noise levels, better balanced alleles, and higher quality results compared to a one-tube approach. This means more reliable typing results. For the amplification of HLA-A, -B, -C, -DRB1, -DQB1 and –DPB1, DRB3/4/5, DQA1, and DPA1. A ready-to-use PCR mastermix (GenDx-LongMix) is included in the kit.

Compatible with Illumina and PacBio sequencing systems.

Available as RUO, CE-marked IVD (IVDR), and Health Canada IVD.

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3 Items

Product Status Reactions Catalog

NGSgo®-MX11-3

Components

NGSgo-MX11-3 primer Mix A

NGSgo-MX11-3 primer Mix B

NGSgo-MX11-3 primer Mix C

NGSgo-MX11-3 Buffer A

NGSgo-MX11-3 Buffer B

NGSgo-MX11-3 Buffer C

GenDx-LongMix

 IVD Canada 96 7771864 Favorite

NGSgo®-MX11-3

Components

NGSgo-MX11-3 primer Mix A

NGSgo-MX11-3 primer Mix B

NGSgo-MX11-3 primer Mix C

NGSgo-MX11-3 Buffer A

NGSgo-MX11-3 Buffer B

NGSgo-MX11-3 Buffer C

GenDx-LongMix

Nuclease-free H2O

 CE-IVD 96 7971864 Favorite

NGSgo®-MX11-3

Components

NGSgo-MX11-3 primer Mix A

NGSgo-MX11-3 primer Mix B

NGSgo-MX11-3 primer Mix C

NGSgo-MX11-3 Buffer A

NGSgo-MX11-3 Buffer B

NGSgo-MX11-3 Buffer C

GenDx-LongMix

Nuclease-free H2O

 RUO 96 7371864 Favorite

Specifications

NGSgo-MX11-3 is fast and easy to use, as multiplexing means less pipetting and pooling. Pipetting is further minimized by our GenDx-LongMix enzyme mastermix, which is included in the kit. The product is compatible with the NGSgo workflow, for a library prep with minimal cleanups and quantifications.

With NGSgo-MX11-3 you get from DNA to report in only 1,5 days. Start your amplification on day 1 and have your typing report on day 2 before noon. You can run 16 samples on an Illumina micro flow cell.

HLA locus-specific amplification is performed in a thermal cycler using the primer mix, DNA, enzyme mastermix, and water. The resulting HLA locus-specific amplicons can be used for identification of HLA alleles by means of Next-generation sequencing.

 

Amplification coverage

 

To be supplied by the user:

  • Cold block or ice
  • Pipettes and pipette tips with hydrophobic filters
  • Thermal cycler
  • Microcentrifuge

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