Which NGS products are available for HLA typing?

HLA sequencing using short reads:

  • NGSgo-AmpX v2/3 for single locus amplification of HLA-A/B/C/DRB1/DRB3/ DRB4/DRB5/DQA1/DQB1/DPA1/DPB1 and HLA-G (NGSgo-AmpX v2).
  • NGSgo-MX6-1 for multiplex amplification of HLA-A/B/C/DRB1/DQB1/DPB1
  • NGSgo-MX11-3 for multiplex amplification of HLA-A/B/C/DRB1/DRB3/ DRB4/DRB5/DQA1/DQB1/DPA1/DPB1
  • NGSgo Library Full Kit for library preparation compatible with above mentioned amplification strategies

HLA sequencing using long reads:

  • NGS-Turbo: NGS-TurboAmp & NGS-TurboPrep for multiplex amplification & library preparation of HLA-A/B/C/DRB1/DRB3/DRB4/DRB5/DQA1/DQB1/DPA1/DPB1
    • HLA typing under 3h
  • NGS-Pronto: NGSgo-ProntoAmp & NGS-ProntoPrep for multiplex amplification amplification & library preparation of HLA-A/B/C/DRB1/DRB3/ DRB4/DRB5/DQA1/DQB1/DPA1/DPB1

Software for analysis of sequencing data:

  • NGSengine for NGSgo-AmpX v2/3, NGSgo-MX6-1 and NGSgo-MX11-3
  • NGSengine-Turbo for NGS-Turbo and NGS-Pronto