When a new update is available, please install via the following link:

https://gendx.com/sbtengine-software

 

 

RELEASE NOTES

 

Version 3.41.0 Release – July 2024

New features

Major

1.      IMGT/HLA database 3.56.0 is included and selected by default.

 

Bug fixes

2.      None.

 

Safety and security impact

3.      None.

 

IFU updates

4.      The Instructions for use (IFU) is frequently updated and provided in multiple languages.

5.      The latest versions can be downloaded from here: www.gendx.com/ifu

 

 

Version 3.40.0 Release – January 2024

New features

Major

1.      IMGT/HLA database 3.54.0 is included and selected by default.

 

Bug fixes

2.      None.

 

Safety and security impact

3.      None.

 

IFU updates

4.      The Instructions for use (IFU) is frequently updated and provided in multiple languages.

5.      The latest versions can be downloaded from here: www.gendx.com/ifu

 

 

Version 3.39.0 Release – 1stof June 2023

New features

Major

1.      IMGT/HLA database 3.52.0 is included and selected by default.

2.      Samples with many genotype ambiguities (e.g. >50,000) will be loaded faster due to speed improvements.

 

Minor

3.      The order of alleles in the ‘All Samples’ report, which can be found under the ‘Typing Result’ and the ‘Approved Loci’ tab, has been improved and sorted on expected frequency and CWD status.

 

Bug fixes

4.      None.

 

Safety and security impact

5.      None.

 

IFU updates

6.      The Instructions for use (IFU) is frequently updated.

7.      The latest versions can be downloaded from here: www.gendx.com/ifu

 

 

Version 3.38.0 Release – 28thof February 2023

New features

1.      Latest IMGT/HLA database, release 3.51.0, is included.

 

Bug fixes

2.      None.

 

Safety and security impact

3.      None.

 

IFU updates

4.      The Instructions for use (IFU) is frequently updated.

5.      The latest versions can be downloaded from here: www.gendx.com/ifu

 

 

Version 3.37.0 Release – 12thof December 2022

New features

1.      Latest IMGT/HLA database, release 3.50.0, is included.

 

Bug fixes

2.      In rare cases, SBTengine would crash when a trace contained too many N’s, now a warning will be shown instead.

 

Safety and security impact

3.      None.

 

IFU updates

4.      The Instructions for use (IFU) is frequently updated.

5.      The latest versions can be downloaded from here: www.gendx.com/ifu

 

 

Version 3.36.0 Release – 5thof October 2022

New features

1.      Latest IMGT/HLA database, release 3.49.0, is included.

 

Bug fixes

2.      The offline help and the help in the glossary are removed. Users can still access the online help in the “Help” menu.

 

Safety and security impact

3.      None.

 

IFU updates

4.      The Instructions for use (IFU) is frequently updated.

5.      The latest versions can be downloaded from here: www.gendx.com/ifu

 

 

Version 3.35.0 Release – 27thof June 2022

New features

1.      Latest IMGT/HLA database, release 3.48.0, is included.

 

Bug fixes

2.      Fixed a bug where the ‘Ask for support’ feature did not work with more recent versions of Outlook mail.

3.      The aberrant splice site for some HLA-G Q-alleles was not recognized, this is corrected within IMGT/HLA 3.48.0.

 

Safety and security impact

4.      None.

 

IFU updates

5.      The Instructions for use (IFU) is frequently updated.

6.      The latest versions can be downloaded from here: www.gendx.com/ifu

 

 

Version 3.34.0 Release – 4th of April 2022

New features

1.      Latest IMGT/HLA database, release 3.47.0, is included.

 

Bug fixes

2.      None

 

Safety and security impact

3.      None

 

IFU updates

4.      The Instructions for use (IFU) is frequently updated.

5.      The latest versions can be downloaded from here: www.gendx.com/ifu

 

 

Version 3.33.0 Release – 15th of December 2021

New features

1.      Latest IMGT/HLA database, release 3.46.0, is included.

 

Bug fixes

2.      None

 

Safety and security impact

3.      None

 

IFU updates

4.      The Instructions for use (IFU) is frequently updated.

5.      The latest versions can be downloaded from here: www.gendx.com/ifu

 

 

Version 3.32.0 Release – 10th of November 2021

New features

1.      Latest IMGT/HLA database, release 3.45.1, is included.

 

Bug fixes

2.      Fixed a bug where some HLA-DPB1 GSSPs were not aligned when using IMGT/HLA 3.37.0 or higher.

 

Safety and security impact

3.      None

 

IFU updates

4.      The Instructions for use (IFU) is frequently updated.

5.      The latest versions can be downloaded from here: www.gendx.com/ifu

 

 

Version 3.31.0 Release – 5th of August 2021

New features

1.      Latest IMGT/HLA database, release 3.44.1, is included.

2.      The splash screen shown upon startup has a new design.

3.      Discontinued the GPS tab for IMGT/HLA 3.44.1 and future IMGT/HLA versions because of memory issues. The tab is still available for older IMGT/HLA versions.

 

Bug fixes

4.      Fixed a bug where updating the NMDP codes resulted in a crash due to memory issues.

 

 

Version 3.30.0 Release – 30th of March 2021

New features

1.      Latest IMGT/HLA database, release 3.43.0, is included.

2.      New splice sites have been added to HLA-A, -B, -C, -E, -F, -G, DRB1, DRB3/4/5, DPA1, DPB1, DQA1, DQB1, MICA and MICB when using IMGT/HLA 3.43.0 to the analyzed region of SBTengine, which can resolve ambiguities, including A*11:01 with A*11:347N. 

 

Bug fixes

3.      Case-sensitive LIMS systems could not read the upper case .XML extension of exports, as present in SBTengine 3.29.0 only. This has been corrected and changed into the lower case .xml extension.

 

 

Version 3.29.0 Release – 8th of January 2021

New features

1.      Latest IMGT/HLA database, release 3.42.0, is included.

2.      Improved performance.

3.      DRB3/4/5 GSSP R7 is recognized correctly

 

Version 3.28.0 Release – 19th of October 2020

New features

1.      Latest IMGT/HLA database, release 3.41.0, is included.

2.      Improved performance.

3.      HLA-B*07:386N that is reported as an ambiguity when using IMGT/HLA database 3.39.0 and 3.40.0 can now be excluded when using IMGT/HLA 3.41.0.

 

Bug fixes

4.      Fixed a bug where the DRB4*01:03:01:13N allele was not reported as an ambiguity of DRB4*01:03:01:02N when using the optimized typing setting.

5.      The csv export file of the sample overview did not show the locus names, which has been corrected.

6.      SBTengine now automatically recognizes the SBTexcellerator R7 primer as a GSSP for DRB3/4/5.

 

Version 3.27.0 Release – 30th of June 2020

New Features

1.      Latest IMGT/HLA database release, version 3.40.0 is included

 

 

Version 3.26.0 Release – 13th of May 2020

New Features

1.      Latest IMGT/HLA database release, version 3.39.0 is included

 

Version 3.25.1 Release – 31st of January 2020

Bug fix

1.      Long ambiguity lists will no longer cause the software to stop the analysis.

 

 

Version 3.25.0 Release - 10 December 2019

New Features

1.      Latest IMGT/HLA database release, version 3.38.0 is included

2.      The NMDP has recently changed the location of the most recent NMDP code list. SBTengine now uses this new link to update the NMDP codes.

 

 

Version 3.24.0 Release – 10 September 2019

New Features

1.      Latest IMGT/HLA database release, version 3.37.0 is included

 

 

Version 3.23.0 Release – 8 August 2019

New Features

1.      Latest IMGT/HLA database release, version 3.36.0 is included

 

Bug fixes

1.      Several small bugs were fixed

 

 

Version 3.22.0 Release – 26 February 2019

New Features

1.      Latest IMGT/HLA database release, version 3.35.0 is included

 

 

Version 3.21.0 Release – 17 December 2018

New Features

1.      Latest IMGT/HLA database release, version 3.34.0 is included

 

 

Version 3.20.1 Release – 27 September 2018

Bug Fix

1.      Expanded HLA-C analysis regions to recognize the C*07:02:01:17N null allele

 

Version 3.20.0 Release – 12 September 2018

New Features

1.      It is now possible to generate an rtf report, readable with Word, with the typing of each locus of a sample on separate pages.

2.      It is now possible to include customized text in the header of the RTF report, such as the name of the institute.

3.      Latest IMGT/HLA database release, version 3.33.0 is included

 

Bug fixes

4.      Several small bugs were fixed

 

 

Version 3.19.1 Release – 16 July 2018

New Features

1.      Expanded HLA-C analysis regions to recognize the C*07:02:01:17N null allele

2.      Bug fix: the checked and unchecked crucial positions are marked differently

 

Version 3.19.0 Release – 4 July 2018

New Features

1.      Latest IMGT/HLA database release, version 3.32.0 is included

2.      The performance has been improved for specific cases in which the ambiguity lists became very long.

 

Version 3.18.1 Release – 5 April 2018

Bug fixes

1.      At startup a warning concerning a deprecated licensing system was shown in some cases.

 

 

Version 3.18.0 Release – 3 April 2018

New Features

1.      Latest IMGT/HLA database release, version 3.31.0.

2.      HLA-E and HLA-F were added to the database and can now be analyzed in SBTengine.

3.      Added possibility to save an extra copy of the XML after first review to a second location for use in LIMS.

4.      Expanded HLA-C analysis regions to recognize the C*15:02:01:08N null allele.

 

Bug fixes

5.      GSSPs are now always recognized.

 

 

Version 3.17.0 Release - 20 November 2017

New Features

1.      Latest IMGT/HLA database release, version 3.30.0.

Bug fixes

2.      DPB1*04:01:01:24N, added to IMGT/HLA database 3.29.0, can now be resolved.

 

 

Version 3.16.1 Release - 28 September 2017

Bug fixes

1.      Locus information in the sequence overview tab is now displayed properly.
The issue of incorrect locus representation was specific for SBTengine 3.16.0.

 

 

Version 3.16.0 Release - 21 September 2017

New Features

1.      Latest IMGT/HLA database release, version 3.29.0.

Bug fixes

2.      Latest Q alleles, which were added in IMGT/HLA database 3.28.0, can now be resolved.

3.      Improvement on the assignment of NMDP codes.

 

 

Version 3.15.0 Release- 17 May 2017

New Features

1.      Latest IMGT/HLA database release, version 3.28.0.

2.      The desktop icon has been re-designed.

Bug fixes

3.      DRB5*01:08N is now correctly identified (issue is present with IMGT/HLA database 3.27.0).

4.      Rarely occurring extremely high peaks do not result in a ‘range check error’ anymore.

 

 

Version 3.14.0 Release- 22 February 2017

New Features

1.      Latest IMGT/HLA database release, version 3.27.0

2.      The use of GSSPs can individually be selected. Lot numbers of reagents are not supported anymore.

3.      Improved advice on resolving strategies in case of incomplete IMGT/HLA information.

 

 

Version 3.13.0 Release- 09 November 2016

New Features

1.      Latest IMGT/HLA database release, version 3.26.0

 

 

Version 3.12.0 Release- 07 September 2016

New Features

1.      New IMGT/HLA database release, version 3.25.0

2.      Ask for Support window: log files are added to the zip file.

 

 

Version 3.11.0 Release- 12 July 2016

New Features

1.      New IMGT/HLA database release, version 3.24.0.1

2.      GSSP recommendation strategy has been optimized.

3.      HLA-A*31:01:02 and null allele A*31:01:02:03N can now be discriminated.

4.      Intuitive reporting of allele ambiguities.

5.      Additional xml reports can automatically be created after approval by second reviewer.

6.       Logging of SBTengine actions can be enabled to facilitate trouble shooting.

Bug fixes

7.      SBTengine now also properly handles ab1 files without content.

8.      Improvement to reporting of sequence summary of DRB3, DRB4 and DRB5 genes.

9.      Some other small bugs were fixed.

 

 

Version 3.10.0 Release- 9 February 2016

New Features

1.      New IMGT/HLA database releases, version 3.22.0 and 3.23.0

2.      Quality Overview can be exported in text or excel format.

3.      Changes have been made to the user interface to show that AlleleSEQR is now a trademark of GenDx.

4.      Several improvements have been included to advice the best ambiguity resolving strategy.

Bug fixes

5.      Several improvements in management of license keys have been made.

6.      Some other small bugs were fixed.

 


Version 3.9.0 Release- 8 October 2015

New Features

1.      New IMGT/HLA release 3.21.1

2.      A quality statistics overview can now be generated of all approved samples.

3.      Font sizes in the typing report can be adjust by the user.

4.      Interpretation of DRB5*01:08N allele is done correctly.

Bug fixes

5.      Several improvements in management of license keys have been made.

 


Version 3.8.0 Release- 16 June 2015

New Features

1.      New IMGT/HLA release 3.20.0

2.      Implementation of a messaging system warning when the license almost expires, or is expired.

3.      HLA Typing resolution can now also be restricted to second or third field resolution.

4.      Old NMDP nomenclature (V2) discontinued.

5.      Updated report section for better presentation of approved typing results.

6.      Improved representation of mismatch levels in the crucial position list.

Bug fixes

7.      Reordering of traces or adjusting trace intensity no longer reset after making edits.

8.      Settings for adjusted coloring of quality values was not stored.

9.      Correct error handling in case trace files do not contain sequence data.

 


Version 3.7.0 Release- 26 March 2015

New Features

1.      New IMGT/HLA release 3.19.0

2.      Updated allele frequency lists presenting more accurate allele frequencies for DQB1*02:01 and DQB1*02:02.

3.      Option to ignore silent/intron variants in the GPS tool.

4.      In the Sample Management Tab, the dropdown menu has been changed into one with radio buttons. Texts have been edited for clarity.

5.      Resolving GSSPs are now presented in a table, which makes the information clearer.

6.      Approval of new alleles is improved.

7.      Amplification strategy has been updated for HLA-DRB1 locus using the AlleleSEQR approach, such that this covers now the entire exon 2 region.

Bug fixes

8.      In some cases, approving of a new allele resulted in a range check error. This has been resolved.

9.      Changing the library location through the preferences is not hampered by refreshing screen.

10.  Some minor bugs are fixed

 

 

Version 3.6.1 Release – 29 January 2015

 New Features

 1.      New IMGT/HLA release 3.18.0

2.      The specificity of a Group Specific Sequencing Primer (GSSP/ARP/MSP) can now also be used for a primer generating a read through sequence in an adjacent exon. This feature enhances resolution for exon 2 - exon 3 cis-trans ambiguities.

3.      For AlleleSEQR users, it is now possible to exclude the amplification primers that are located in the exon regions.

4.      NMDP code selection is further optimized: in case the ambiguities can be represented by multiple NMDP codes, the alleles are combined based on P- and/or G-groups.

5.      The results in Sample Overview can now also be exported as CSV files, next to Excel and PDF.

  Bug fixes

11.  Some minor bugs are fixed

 

 

Version 3.5.2 Release – 18 September 2014

 Bug fixes

1.      This release contains updated versions of the IMGT/HLA databases, including the P- and G group definitions, which were missing in the IMGT/HLA databases released with version 3.5.0.

2.      No other changes were made

 

Version 3.5.0 Release – 10 September 2014

New or adjusted Features

1.      Updated licensing and installation structure, requiring single reactivation of license per computer

2.      New IMGT/HLA release 3.17.0.1

3.      Improved algorithm for better analysis of regions with overlapping base calls

4.      IVD marking for US now indicated on updated splash screen for users in USA

5.      Analysis speed of GPS tool increased

6.      New layout of MSP/GSSP resolving strategies

Fixed bugs

7.      Editor does no longer jump to a different position after making an edit

8.      Refresh issues solved

9.      Some other minor bugs are fixed

 

Version 3.4.1 Release – 16 July 2014

Fixed bugs

1.      The new algorithm released with version 3.4 for alignment and typing seemed to have unforeseen side effects that some sequences are being rejected in case certain peaks are not fully separated. The cause has been identified, corrected and tested and the algorithm has been adjusted accordingly.

2.      No other changes were made

 

Version 3.4.0 Release – 9 July 2014

New or adjusted Features

1.      New algorithm is used for alignment and typing, limiting the manual editing requirements and thereby speeding up the overall process.

2.      Ratio of identified alleles is shown per sequencing run

3.      Sequence read through from one exon to another exon are now linked and will help to solve certain cis-trans ambiguities.

4.      New IMGT/HLA release 3.16.0

5.      Improved management of some alleles with InDels (like C*06:116N)

6.      New HLA allele frequency table are used, as published in 2013 by NMDP

Fixed bugs

7.      Some minor bugs are fixed

 

Version 3.3.0 Release - 20 February 2014

New or adjusted Features

  1. New IMGT/HLA release 3.15.0
  2. Detailed Sequence colour coded quality values are shown in [Sample management] window.
  3. DART® solution, suggesting GSSP/ARP/MSP for ambiguity resolving can now also be shown in table format.
  4. Yellow Hightlighter in [Sequence Overview], to mark the editing postion, cna be turned off/on in [Preferences] / [General].
  5. Faster position checking by jumping to unchecked positions only.
  6. Easy analyses of HLA-C exon 6-7 'read throughs' for multiple reagent strategies.

Fixed bugs

7.      Some minor bugs are fixed

 

Version 3.2.0 Release - 15 November 2013

New or adjusted Features

  1. IMGT/HLA release 3.14.0
  2. Enhanced functionality to confirm/exclude more alleles that have in/dels within exon regions.
  3. Automatic updating now checks weekly for updates
  4. Manual allocation of a GSSP to a sequencing file has been simplified
  5. Sample Report view
    1. Option to list genotypes, P and G groups
    2. P and G groups can be independently shown
    3. Allele frequencies shown
    4. Colour coded CWD status shown
  6. Comprehensive project overview of samples
    1. Listing of detailed results of all samples
    2. Intuitive colour coding of the results
    3. Various options to filter results
    4. Reports can be exported to Excel or PDF
  1. Specific adjustments for Abbott Molecular customers
    1. EDRB1 locus available as of IMGT/HLA release 3.10.0

Fixed bugs

  1. GPS module does not crash with EDRB1 locus
  2. Several minor bugs have been fixed.

 

Version 3.1.0 Release - 29 August 2013

New or adjusted Features

  1. New IMGT/HLA release 3.13.1
  2. CWD 2.0 list implemented, two versions: 1) Common alleles 2 ) Common and well documented alleles
  3. New cloud based licensing system (QLM) with:
    1. Simplified license installation and updating
    2. Option to log in and assign reviewer levels for improved data workflow
    3. New users will automatically make use of this new licencing system and reviewer level functionality. Existing users will gradually be transitioned
  4. New look for ‘Ask for Support’ option
  5. Enhanced GSSP selection tool, allowing the use of additional GSSP lists (optional for a fee)
  6. Software code optimized to include enhanced messaging functionality for exception handling
  7. Several additional minor improvements
  8. Specific adjustments for Abbott Molecular customers
    1. Log in and assign reviewer levels
    2. EDRB1 locus, extended version of the Celera AlleleSEQR® reagents
    3. Selection of Celera Ambiguity Resolving Primers (ARP), including prioritizing ARP’s for DPB1 codon 85 and EDRB1 codon 86
    4. Selection of Celera Motif-specific Sequencing Primers (MSP)

 

Version 2.23.0.0 Release - 17 May 2013

New or adjusted Features

  1. New IMGT/HLA release 3.12.0.
  2. Additional minor improvements have been implemented

Fixed bugs

  1. Several minor bugs have been fixed.

 

Version 2.22.0.0 Release - 6 February 2013

New or adjusted Features

  1. New IMGT/HLA release 3.11.0.
  2. End User License Agreement (EULA) available in About box.

Fixed bugs

  1. Several minor bugs have been fixed.

 

Version 2.21.0.0 Release - 1 November 2012

New or adjusted Features

  1. New IMGT/HLA release 3.10.0.
  2. GPS function has been optimized.
  3. Wizard for new license import has been implemented.

Fixed bugs

  1. Several minor bugs have been fixed.

 

Version 2.20.0.0 Release - 8 August 2012

New or adjusted Features

  1. New IMGT/HLA release 3.9.0.
  2. New IUPAC-IUB Codes diagram hotkey is F2.
  3. SBTengine help also available offline using hotkey F1.
  4. Ambiguity list generation for GPS is faster and requires less RAM.
  5. New features are shown in a pop-up window during startup.

Fixed bugs

  1. Several minor bugs have been fixed.

 

Version 2.19.0.0 Release - 24 May 2012

New or adjusted Features

  1. New IMGT/HLA release 3.8.0.
  2. New GPS (GSSP Prediction Software) tool launched! Based on low-medium resolution HLA typing, GPS determines upfront which GSSPs can be included in SBT to resolve ambiguities. The recommendation is performed based on the active allele frequencies, and the GSSPs availability.

Fixed bugs

  1. Several minor bugs have been fixed.

 

Version 2.18.0.0 Release - 10 April 2012

New or adjusted Features

  1. New IMGT/HLA release 3.7.0.
  2. Search sample functionality for enhanced sample selection.
  3. Supports the use of different SBTexcellerator GSSP sets based on lot numbers.
  4. Glossary displays SBTengine manual.
  5. Release notes directly link to respective item in SBTengine manual.

 

Fixed bugs

  1. Several minor bugs have been fixed.

 

Version 2.17.1.0 Release - 3 November 2011

New or adjusted Features

  1. To overcome possible user specific network obstacles the ‘ask for support’ procedure has been adjusted such that the temporary [zip file] is now stored in user work folder on local or network drive.

 Fixed bugs

  1. XML setting in preferences adjusted such that it is stored after quitting the program.
  2. alignment procedure has been adjusted to overcome no calling in cases where IMGT/HLA-library does not contain any polymorphic position (e.g. exon 6 of HLA-B).
  3. adjusted manual sample naming procedure.
  4. inconsistent GSSP usage suggestion in relation to use/no use of CWD list.
  5. statement was missing “ambiguity across 2-digit group”.

 

Version 2.17.0.0 Release - 14 October 2011

New Features

  1. New IMGT/HLA release 3.6.0
  2. New installation procedure!
    1. solving user account authorization issues
  3. Increased security.
    1. A secure certificate is included to ensure that the files derived from GenDx are not altered.
  1. Alignment procedure has been adjusted to overcome rejection of high quality homozygous sequences
    1. Due to the vast growing number of alleles the consensus sequence in various HLA loci show extended polymorphic areas that hamper proper alignment.
  1. New branding of GenDx features, logo, colours etc.
  2. Additional minor improvements have been implemented

 Fixed bugs

  1. Several minor bugs have been fixed.

 

Version 2.16.1.0 Release - 10th June 2011

Fixed bugs

  1. [Unable to create directory] error upon approval in version 2.16.0.0 is fixed
  2. Sample selection of manually assigned sample names/loci bug in version 2.16.0.0 is fixed

 

Version 2.16.0.0 Release - 25th May 2011

New Features

  1. New IMGT/HLA release 3.4.0
  2. Quality Value filtering for crucial positions
  3. CWD list(s) filtering for crucial positions
  4. Allele presence on CWD list indicated
  5. Allele frequencies for sorting crucial positions
  6. Fully editable allele frequency lists
  7. Expected genotype frequency of typing result
  8. P and G Codes for reporting ambigious allele typings
  9. Autoload next sample/locus after approval
  10. Improved layout Sequence Overview
  11. Additional minor improvements have been implemented

 

Version 2.15.0.0 Release - 2nd February 2011

New Features

  1. New IMGT/HLA release 3.3.0
  2. Additional minor improvements have been implemented

 

Version 2.14.0.0 Release - 10th November 2010

New Features

  1. New IMGT/HLA release 3.2.0
  2. Specificity of GSSPs can be used to enhance Ambiguity Resolving Power
  3. Improved horizontal scaling of the sequence trace
  4. Reports can be automatically saved as RTF files
  5. Additional minor improvements have been implemented

 

Version 2.13.0.0 Release - 24th August 2010

New Features

  1. New IMGT/HLA release 3.1.0
  2. Resizable window panels in Sequence Overview & Sample Management
  3. Easy selection of work folder history in Sample Management
  4. Crucial positions list in Sequence Overview shows near-matching genotypes
  5. Enhanced ambiguity-resolving power by improved GSSP selection
  6. Additional minor improvements have been implemented

 

Version 2.12.1.0 Release - 23rd April 2010

New Features

  1. New IMGT/HLA release 3.0.0
  2. New HLA nomenclature has been implemented.
  3. Additional minor improvements have been implemented

 

Version 2.11.1.0 Release - 14th February 2010

New Features

  1. A licensing problem associated with systems utilizing Asian characters has been solved
  2. Additional minor improvements have been implemented

 

Version 2.11.0.0 Release - 28th January 2010

New Features

  1. New IMGT/HLA release 2.28.0
  2. Improved analysis of non-overlapping GSSPs.
  3. Accepts international characters in folder paths and file names.
  4. Additional minor improvements have been implemented.

 

Version 2.10.0.0 release 13 November 2009

New Features

  1. New IMGT/HLA release 2.27.0
  2. NMDP code now available in typing report. This function must be activated by the user (see NMDP codes topic in online help).
  3. In crucial position listview, clicking on column header sorts data in that column.
  4. When using 'Ask for Support' button, the generated ZIP files can now be found in the 'GenDx Support' folder on the desktop.

Fixed bugs

  1. Several minor bugs have been fixed.

 

Version 2.9.0.0 release 28 August 2009

New Features

  1. New IMGT/HLA release 2.26.0
  2. Enhanced formulation of ambiguity resolving strategies.
  3. Ambiguity resolving strategies included in report and XML file.
  4. Improved GSSP selection by optimized GSSP ranking analysis.
  5. Many technical 'under the hood' features have been improved to give you a better SBTengine experience.

Fixed bugs

  1. Several minor bugs were fixed.

 

Version 2.8.0.0 release 15 May 2009

New Features

  1. CE IVD marked!
  2. New IMGT/HLA release 2.25.2
  3. Exons are proportionally displayed in the Sequence Overview window.
  4. Enhanced interpretation of homozygous and heterozygous sequence traces.
  5. Improved new allele reporter; now includes available GSSP data.
  6. Sequence trace basecalling can be reset.
  7. KIR, DLA, HA-1 and MICA libraries available on request.

Fixed bugs

  1. Several minor bugs were fixed.

 

Version 2.7.0.1 release 30 January 2009

New Features

  1. New alleles can be reported!
  2. New IMGT/HLA release 2.24
  3. Improved 'Ask for support' functionality to give you even better support.
  4. Switching between workfolder and archive can be done directly in the 'Sequence Overview' window.
  5. Genotypes containing an IMGT-incomplete sequence are indicated.

Fixed bugs

  1. Several minor bugs were fixed.

 

Version 2.6.0.0 release 12 November 2008

New Features

  1. New IMGT/HLA release 2.23.0
  2. HLA-DQA1 analysis is now possible, with correct management of the codon 56 InDel.
  3. HLA-DPA1 analysis in now possible.
  4. All comments entered at approval, will be maintained and reported.
  5. Improved basecalling and alignment.
  6. Sequences now can be aligned with more than 3 regions.
  7. Heterozygote positions only are listed once in the ‘heterozygous positions’ representation.
  8. Better recognition of dye-blobs.

Fixed bugs

  1. Several minor bugs were fixed.

 

Version 2.5.0.0 release 19 August 2008

New Features

  1. Improved basecalling
  2. New IMGT/HLA library, release 2.22
  3. Sequences are sorted in Sequence Overview
  4. More robust alignment of sequences with IMGT/HLA library
  5. Typing report shows all relevant version details of SBTengine
  6. Sequences can be printed from the Sequence Editor

Fixed bugs

  1. Several minor bugs were fixed

 

Version 2.4.0.0 release 28 April 2008

New Features

  1. Support button included, when used a ZIP file for current sample, including all sequencing files, is automatically created and may be send to GenDx team for review and support.
  2. New IMGT/HLA library, release 2.21
  3. Handling of DRB region is significantly altered
    • DRB locus is analysed separately. Either HLA-DRB1 or HLA-DRB345 is selected. Analyses method is now identical to the one used for other loci.
    • GenDx GSSP with specificity for codon 86 maybe selected to include in analyses. This will reduce the number of ambiguities
    • XML creation for DRB345 is changed. For each locus, a separate XML file is generated.
  4. Added several features to improve functionalities for GSSP
    • Recognize GSSPs automatically when present in file name or comment. Currently only HLA-DRB1 Codon86 is included.
    • GSSPs are highlighted when a typing result is obtained
    • Displaying usage of GSSP specificity
    • Added support to alter GSSP specificity
  5. Display modifications
    • A track bar was added to each chromatogram to allow vertical scaling
    • allowed to move sequences up or down in sequence overview
    • allowed to add a comment into .xml file
  6. other new features
    • Show IMGT/HLA details when download fails
    • Show XML comment in lab report,
    • Show "request license" when no valid license is found
    • Request license button in ‘about box’
    • In list of crucial position a single mismatch (1 MM) remains to be shown at top, irrespectively whether 2 MM and 3 MM are checked or not

Fixed bugs

  1. Several minor bugs were fixed

 

Version 2.3.0.0 release 22 January 2008

New Features

  1. New IMGT/HLA library, release 2.20
  2. All HLA null-alleles can be excluded (except DRB4*0301) and several null alleles, but also alleles with designators L, S and Q, can even be positively confirmed!
  3. Handling of multiple intron sequences during analyses is streamlined
  4. Blocking of fire-walls reduced by allowing to logon to a institute proxy server with a user defined username and password. This will enhance automatic updating functionalities
  5. List of unchecked crucial positions, shown in bold, now shows at top of the list
  6. The "look" of SBTengine has been further adjusted according to Windows presentation modes

Fixed bugs

  1. Several minor bugs were fixed

 

Version 2.2.0.0 release 24 October 2007

New Features

  1. New IMGT/HLA library, release 2.19
  2. By default all readthrough sequences are disabled for allele assignment after first time alignment
  3. GATC legend now clickable to show or hide chromatogram traces
  4. List of crucial positions, shown in bold, now shows at top the yet unchecked positions
  5. When present, the exact position of a deletion or insertion in a particular sequence is shown
  6. The "look" of SBTengine has been adjusted according to Windows presentation modes

Fixed bugs

  1. Several minor bugs were fixed

 

Version 2.1.0.0 release 7 September 2007

Fixed bugs

  1. Minor bugs were fixed

 

Version 2.0.0.1 release 23 July 2007

New Features

The software package SBTengine has been improved significantly and enables you to perform your analysis even faster and easier

  1. New IMGT/HLA library, release 2.18
  2. Enhanced Alignment Algorithm opens samples 4 times faster
  3. DART ™ selected GSSP's for DQB1 and DPB1
  4. Details of GSSP primers included in 'Glossary'
  5. Average Phred Quality Scores for sequences included
  6. Amino acid coding triplets are indicated
  7. Colored base calling helps you with analysis
  8. Added menu item and F1 shortcut to show NC-IUB codes
  9. Nucleotides are shown in color
  10. Added print & save buttons to typing result for active locus
  11. Linked jump between Sequence Overview and Sequence Editor
  12. Added sound signal when crucial positions appear
  13. Ambiguities are now considered part of typing result in reports
  14. All full matches are now listed as 'Matches'

Fixed bugs

  1. Several minor bugs were fixed.
  2. Incorrect highlighting of crucial positions was solved
  3. On opening sample, sequences appear consistently in alphabetical order

 

Version 1.25.1.1.511 release 23 April 2007

Major New Features

  1. Updated IMGT/HLA allele library to version 2.17.0
  2. Better integration of editor and sequence overview

Fixed bugs

  1. Several minor bugs were fixed.

 

Version 1.24.1.1.475 release 26 January 2007

Major New Features

  1. Updated IMGT/HLA allele library to version 2.16.0
  2. Improved display of electropherogram
  3. By default, exon 1 and exon 5 are excluded from initial analysis (class I)
  4. DART: Dynamic Ambiguity Resolving Tool to assist selection of the correct sequencing primer for GSSP

Fixed bugs

  1. Several minor bugs were fixed.

 

Version 1.23.1.1.429 release 12 October 2006

Major New Features

  1. Updated IMGT/HLA allele library version 2.15.0
  2. Automatic selection of a Group Specific Sequencing Primer to solve a genotype ambiguity. Suggested GSSP's are present in reagents box SBTexcellerator.
  3. Manual editing of individual sequences is now possible.
  4. Rejected sequences are automatically available for reviewing and editing if required
  5. Original basecalling can be used as is performed by ABI analyses software. Users should use the "'mixed base" mode.
  6. Access to archive data has been optimized.

Fixed bugs

  1. Several minor bugs were fixed.

 

Version 1.22.1.1.425 release 26 July 2006

New Features

- Updated IMGT/HLA allele library version 2.14.0
- Monitoring of inspected crucial positions
- Cleanup of run folders after approval
- Faster startup

 

Version 1.21.1.1.412 release 24 May 2006

Fixed bug

  1. Automatic sample name and locus assignment feature has been adjusted, to secure archived samples.

 

Version 1.20.1.1.412 release 3 May 2006

New Features

  1. Updated IMGT/HLA allele library version 2.13
  2. Automatic sample name and locus assignment, fully configurable
  3. Comprehensive lab report with intuitive graphic overview of sequenced regions
  4. Approval is stamped with date, time and persons name. History of earlier approvals is stored
  5. Reset of locus assignment allowing easy reanalysis
  6. Easy deletion of an individual sequencing file together with it's associates (e.g. files with extension seq or phd.1)
  7. Quick reviewing of results of all typed samples as stored in XML exports and present in active directory
  8. Window for setting the Preferences was extended and adjusted
  9. Several others features were added to the engine to facilitate the assignment process.

Fixed bugs

  1. Several minor bugs were fixed.

Known Issues

  1. MRR may be limited in function for SCF files (Licor Biosciences and Pharmacia ALF).
  2. Modifications of nucleotides will not be saved in AB1 files derived from Macintosh platform or SCF files.

 

Version 1.19.1.1.404 release 3 February 2006

New Features

  1. updated IMGT/HLA allele library version 2.12. has been included
  2. DQB1 typing strategy has been adjusted such that now exon 2 OR exon3 is an essential region. This means that one may obtain an allele typing with sequencing data of only exon 3.

Fixed bugs

  1. Several minor bugs were fixed.

Known Issues

  1. MRR may be limited in function for SCF files (Licor Biosciences and Pharmacia ALF).
  2. Modifications of nucleotides will not be saved in AB1 files derived from Macintosh platform or SCF files.
  3. Although the XML files provide a comprehensive report from SBTengine®, we are working hard to get more alternative report forms that are easier accessible to the average SBTengine user.

 

Version 1.16.1.1.363 release 21 December 2005

New Features

  1. Approximately 25 ‘screen dumps' of known anomalies have been added such that these can be re viewed as visual aid to assist proper nucleotide editing. Users are asked to send samples with common anomalies that are not yet covered by the current set of pictures to mailto:support@GenDx.com. (Just send all the curve files of the samples and mention the relevant location).
  2. New self explanatory icons have been added to various windows.

Improvements

  1. Several other features were adjusted to provide more speed and control to the user

Fixed bugs

  1. Several minor bugs were fixed.

Known Issues

  1. MRR may be limited in function for SCF files (Licor Biosciences and Pharmacia ALF).
  2. Modifications of nucleotides will not be saved in AB1 files derived from Macintosh platform or SCF files.
  3. Although the XML files provide a comprehensive report from SBTengine®, we are working hard to get more alternative report forms that are easier accessible to the average SBTengine user. Users are asked to send their request how they would prefer their report.

 

Version 1.12.1.1.358 release 8 November 2005

New Features

  1. Multi region reading (MRR). A single sequence may encompass a larger region including more than one exon. In most cases the user will get more data with less sequencing reactions. The MRR feature is extremely useful, in particular when a larger amplicon is used as a sequencing template.
  2. The ability to toggling on or off a particular sequence during analysis. The color of the corresponding arrow will change from yellow into blue. This feature allows better understanding of the effect of a particular sequence on allele assignment. It allows the user to skip a sequence file from automated analysis, but the chromatogram is still visible.
  3. User may adjust the location of maps, files, color settings etc.
  4. Naming of the samples is now automated and can be fully customized. SBTengine will recognize the sample name of all selected samples at once (we have succesfully renamed ~1.500 sequencing files without user intervention)
  5. Near allele matches are now clearly indicated with a "tool tip" which becomes active after allele assignment and pops up when the mouse hovers over the table with crucial positions.

Improvements

  1. Many alleles differ only at the DNA level, without causing a change at the amino acid level. This also includes differences in the intron regions that do not affect the splicing event. A user may toggle whether to see these types of silent/intron ambiguities or not.
  2. After approving the allele assignment the user is requested whether to review the XML file. The user may toggle this question on or off.
  3. Older Pharmacia ALF files may be exported as Staden SCF files in the "upright" mode. These files can now be analyzed and modified by SBTengine.
  4. IMGT/HLA sequence database version 2.11 has been included
  5. DRB1, DRB3,4,5 typing has been streamlined.
  6. Webhelp manual has been adjusted
  7. Several other features were adjusted to provide more speed and control to the user

Fixed bugs

  1. Selection of nucleotide position has been adjusted such that the cursor now will jump to the position according to the IMGT/HLA database. Negative numbers will be considered as belonging to the intron region.
  2. Last visited position is now functioning.
  3. Several minor bugs were fixed.

Known Issues

  1. MRR may be limited in function for SCF files (Licor Biosciences and Pharmacia ALF).
  2. Although the XML files is a comprehensive report from SBTengine®, we are working hard to get more alternative report forms that are easier accessible to the average SBTengine user. We expect to have alternatives reports available for the end of year 20o5. Users are asked to send their request how they would prefer their report.